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Methotrexate exacerbates NAFLD by inhibiting antioxidant activity, boosting lipid buildup, and generating mitochondrial dysfunction, which leads to increased hepatocyte apoptosis and inflammation

The simulations suggested that quinone metabolism yielded an increase in ROS (O 2 . and H 2 O 2 ), producing a maximum of 0.42 M and 1 10 3 M respectively

Furthermore, MD revealed decreased flexibility of p53 TAD2 in complex with FOXO4-DRI compared to the FOXO4 FH -bound state with RMSDs of 1 heteronuclear NOE data was recorded using the HSQCNOEF3GPSI bruker pulse sequence (32 scans, 256 points in F1, 1024 points in F2, 1278.772 Hz spectral width in F1, 9615.385 Hz spectral width in F2) using a three seconds saturation period

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